pIS0 - miR-196a-as
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12539||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBartel Lab
- Backbone size w/o insert (bp) 5260
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)22
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev primer (Common Sequencing Primers)
Firefly luciferase reporter with perfect antisense complementarity to miR-196a. Insert sequence: 5'-gagctcCCCAACAACATGAAACTACCTAtctaga - 3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIS0 - miR-196a-as was a gift from David Bartel (Addgene plasmid # 12539 ; http://n2t.net/addgene:12539 ; RRID:Addgene_12539)
For your References section:MicroRNA-directed cleavage of HOXB8 mRNA. Yekta S, Shih IH, Bartel DP. Science. 2004 Apr 23. 304(5670):594-6. 10.1126/science.1097434 PubMed 15105502