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PCD FL4X
(Plasmid #12565)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12565 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Luciferase ; Reporter for translation repression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Four CXCR4 imperfect binding sites
  • Alt name
    CXCR4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    100
  • Entrez Gene
    CXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS)
  • Tag / Fusion Protein
    • luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer BGH rev primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. Four imperfect complementary binding sites to the antisense strand of CXCR4 siRNA were cloned in the XbaI/ApaI sites in the 3'UTR of luciferase.

Author's sequence data was obtained by using a primer reading forward from inside the luciferase gene.

Imperfect binding sequence is - 5'-AAGTTTTCACAAAGCTAACA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCD FL4X was a gift from Carl Novina (Addgene plasmid # 12565 ; http://n2t.net/addgene:12565 ; RRID:Addgene_12565)
  • For your References section:

    Recapitulation of short RNA-directed translational gene silencing in vitro. Wang B, Love TM, Call ME, Doench JG, Novina CD. Mol Cell. 2006 May 19. 22(4):553-60. 10.1016/j.molcel.2006.03.034 PubMed 16713585