Purpose(Empty Backbone) B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125744||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMouse Targeting
Growth in Bacteria
Copy numberLow Copy
A portion of this plasmid was derived from a plasmid made byDTA negative selection cassette is from Philippe Soriano. p15A backbone is from Sen Wu, Capecchi laboratory.
Terms and Licenses
Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
AscI and AsiSI (PacI-compatible) cloning sites in the XbaI site of ROSA26 (Soriano, 1999), a highly recombinogenic locus.
The 5' arm appears to have SNP's that differ between 129 and B6 genomes. Please see pROSA26-1 (#21714) and the reference B6J genome.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB6-ROSA26-DTA-Capecchi-rev was a gift from Mario Capecchi (Addgene plasmid # 125744 ; http://n2t.net/addgene:125744 ; RRID:Addgene_125744)