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pB6-ROSA26-DTA-Capecchi-rev
(Plasmid #125744)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125744 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Resource Information

Depositor Comments

Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

AscI and AsiSI (PacI-compatible) cloning sites in the XbaI site of ROSA26 (Soriano, 1999), a highly recombinogenic locus.

The 5' arm appears to have SNP's that differ between 129 and B6 genomes. Please see pROSA26-1 (#21714) and the reference B6J genome.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-ROSA26-DTA-Capecchi-rev was a gift from Mario Capecchi (Addgene plasmid # 125744 ; http://n2t.net/addgene:125744 ; RRID:Addgene_125744)