Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Destination Vector
(Plasmid #125754)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125754 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber
  • Backbone size w/o insert (bp) 1662
  • Total vector size (bp) 3635
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTDH3-GFPdropout-tTDH1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1973

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Destination Vector was a gift from Rodrigo Ledesma-Amaro (Addgene plasmid # 125754 ; http://n2t.net/addgene:125754 ; RRID:Addgene_125754)
  • For your References section:

    Rapid Assembly of gRNA Arrays via Modular Cloning in Yeast. McCarty NS, Shaw WM, Ellis T, Ledesma-Amaro R. ACS Synth Biol. 2019 Apr 2. doi: 10.1021/acssynbio.9b00041. 10.1021/acssynbio.9b00041 PubMed 30939239