Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCMV-AR-R405S
(Plasmid #126051)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126051 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AR
  • Alt name
    androgen receptor
  • Species
    H. sapiens (human)
  • Mutation
    R405S
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The human AR N-terminal glutamine repeat may undergo minor expansion or contraction during bacterial propagation. It may be advisable to limit the time of bacterial growth and to verify glutamine repeat length using Sanger sequencing. Minor changes in AR N-terminal glutamine repeat length should not significantly alter AR activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-AR-R405S was a gift from Elizabeth Wilson (Addgene plasmid # 126051 ; http://n2t.net/addgene:126051 ; RRID:Addgene_126051)
  • For your References section:

    Androgen receptor exon 1 mutation causes androgen insensitivity by creating phosphorylation site and inhibiting melanoma antigen-A11 activation of NH2- and carboxyl-terminal interaction-dependent transactivation. Lagarde WH, Blackwelder AJ, Minges JT, Hnat AT, French FS, Wilson EM. J Biol Chem. 2012 Mar 30;287(14):10905-15. doi: 10.1074/jbc.M111.336081. Epub 2012 Feb 13. 10.1074/jbc.M111.336081 PubMed 22334658