PurposeLabeling of excitatory synapses (green fluorescence) in cre-expressing neurons
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126216||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5603
- Total vector size (bp) 7204
Modifications to backboneInserted CCR5TC DNA binding sequence upstream of promoter.
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)1596
- Promoter EF1A
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
- 3′ sequencing primer CCAGTCAATCTTTCACAAAT (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPart of this gene was derived from Addgene plasmid 46295 deposited by Don Arnold.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC was a gift from Xue Han (Addgene plasmid # 126216 ; http://n2t.net/addgene:126216 ; RRID:Addgene_126216)
For your References section:A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags. Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X. iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330. 10.1016/j.isci.2020.101330 PubMed 32674057