p5343_UC57
(Plasmid #126645)

• Purpose: environmental plasmid p5343 cloned into pUC57
• Depositing Lab: Aindrila Mukhopadhyay
• Publication: Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00588-19. doi: 10.1128/mSystems.00588-19.

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 13545
• Modifications to backbone: None
• Vector type: Synthetic Biology
• Selectable markers: mercury chloride

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Environmental plasmid p5343
• Alt name: p5343_UC57,
• Species: NA
• Insert Size (bp): 8102
• Promoter: NA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: TGAGCACCTTCGGCGACG

Resource Information

• Supplemental Documents:
  • Annotated Sequence
• A portion of this plasmid was derived from a plasmid made by: We isolated the native 8 kb plasmid p5343 from a groundwater sample and synthesized it

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://mbio.asm.org/content/10/1/e02899-18