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pHAGE2-EF1aL-Cre-IRES-NeoR-W
(Plasmid #126700)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126700 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE2
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 7830
  • Vector type
    Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre-IRES
  • Species
    bacteriophage P1, EMCV
  • Insert Size (bp)
    1600
  • Promoter Ef1a
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-EF1aL-Cre-IRES-NeoR-W was a gift from Darrell Kotton (Addgene plasmid # 126700 ; http://n2t.net/addgene:126700 ; RRID:Addgene_126700)
  • For your References section:

    Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells. Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers MF, Kotton DN. Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 10.1016/j.stem.2017.08.014. Epub 2017 Sep 28. 10.1016/j.stem.2017.08.014 PubMed 28965766