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pB6-Hipp11-DTA-Capecchi-rev
(Plasmid #127054)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127054 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    DTA negative selection cassette is from Philippe Soriano. p15A backbone is from Sen Wu, Capecchi laboratory.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

AscI and PacI cloning sites near reported Hipp11 locus (Hippenmeyer et al., 2010), a highly recombinogenic locus.

Genomic sequence in pHipp11 plasmids #52544 and #52545 might be from 129/SvJ mouse according to the paper cited above.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB6-Hipp11-DTA-Capecchi-rev was a gift from Mario Capecchi (Addgene plasmid # 127054 ; http://n2t.net/addgene:127054 ; RRID:Addgene_127054)