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pAAV-CAG-DIO-ChR2(H134R)-eYFP
(Plasmid #127090)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127090 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Alt name
    hChR2(H134R)
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1662
  • Mutation
    Humanized ChR2 gene with histidine 134 changed to arginine (H134R), to achieve higher currents
  • Promoter CAG
  • Tag / Fusion Protein
    • eYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

*Note: This construct is based on Addgene ID 20298 (the Ef1a promoter was changed to the CAG promoter).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-ChR2(H134R)-eYFP was a gift from Viviana Gradinaru (Addgene plasmid # 127090 ; http://n2t.net/addgene:127090 ; RRID:Addgene_127090)
  • For your References section:

    Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, Deverman BE, Munzberg H, Ardell JL, Salama G, Gradinaru V, Shivkumar K. Nat Commun. 2019 Apr 26;10(1):1944. doi: 10.1038/s41467-019-09770-1. 10.1038/s41467-019-09770-1 PubMed 31028266