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pLKO.1-puro-cGASsh4
(Plasmid #127646)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127646 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cGAS shRNA
  • gRNA/shRNA sequence
    CAACTACGACTAAAGCCATTTCTCGAGAAATGGCTTTAGTCGTAGTTG
  • Species
    H. sapiens (human)
  • Entrez Gene
    CGAS (a.k.a. C6orf150, MB21D1, h-cGAS)

Cloning Information

  • Cloning method Unknown

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-puro-cGASsh4 was a gift from Nicolas Manel (Addgene plasmid # 127646 ; http://n2t.net/addgene:127646 ; RRID:Addgene_127646)
  • For your References section:

    Bloom syndrome protein restrains innate immune sensing of micronuclei by cGAS. Gratia M, Rodero MP, Conrad C, Bou Samra E, Maurin M, Rice GI, Duffy D, Revy P, Petit F, Dale RC, Crow YJ, Amor-Gueret M, Manel N. J Exp Med. 2019 May 6;216(5):1199-1213. doi: 10.1084/jem.20181329. Epub 2019 Apr 1. 10.1084/jem.20181329 PubMed 30936263