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pTRIPZ shcGAS-Mmu
(Plasmid #127698)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127698 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRIPZ RFP+
  • Backbone size w/o insert (bp) 13320
  • Total vector size (bp) 13412
  • Modifications to backbone
    insertion of mouse cGAS specific shRNA hairpin sequence between XhoI and Eco RI restriction sites
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shcGAS Mmu
  • Alt name
    Mb21d1, E330016A19Rik
  • gRNA/shRNA sequence
    CAGGATTGAGCTACAAGAATAT
  • Species
    M. musculus (mouse)
  • GenBank ID
    214763
  • Entrez Gene
    Cgas (a.k.a. E330016A19Rik, Mb21d1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site xhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cagaaggctcgagaaggtatattgctgttgctgttgacagtgagcg
  • 3′ sequencing primer ctaaagtagccccttgaattccgaggcagtaggca
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIPZ shcGAS-Mmu was a gift from Sandra Demaria (Addgene plasmid # 127698 ; http://n2t.net/addgene:127698 ; RRID:Addgene_127698)
  • For your References section:

    DNA exonuclease Trex1 regulates radiotherapy-induced tumour immunogenicity. Vanpouille-Box C, Alard A, Aryankalayil MJ, Sarfraz Y, Diamond JM, Schneider RJ, Inghirami G, Coleman CN, Formenti SC, Demaria S. Nat Commun. 2017 Jun 9;8:15618. doi: 10.1038/ncomms15618. 10.1038/ncomms15618 PubMed 28598415