pEYmRD3A
(Plasmid
#127756)
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PurposeResearch the effect of gene orientation of transcription
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBEST
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemSpinach,RFP Divergent Orientation
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning method: Golden Gate. Genbank Accession No. KX682222 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEYmRD3A was a gift from Richard Murray (Addgene plasmid # 127756 ; http://n2t.net/addgene:127756 ; RRID:Addgene_127756) -
For your References section:
Biophysical Constraints Arising from Compositional Context in Synthetic Gene Networks. Yeung E, Dy AJ, Martin KB, Ng AH, Del Vecchio D, Beck JL, Collins JJ, Murray RM. Cell Syst. 2017 Jul 26;5(1):11-24.e12. doi: 10.1016/j.cels.2017.06.001. Epub 2017 Jul 19. 10.1016/j.cels.2017.06.001 PubMed 28734826
