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Addgene

pMH-HT_ygiF-TTM
(Plasmid #127794)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127794 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMH-HT
  • Backbone manufacturer
    Michael Hothorn
  • Backbone size w/o insert (bp) 5345
  • Total vector size (bp) 5951
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ygiF
  • Alt name
    P30871
  • Species
    Escherichia coli
  • Insert Size (bp)
    606
  • Mutation
    N-terminal TTM domain of ygiF (residues 1-200)
  • Entrez Gene
    ygiF (a.k.a. b3054, ECK3044)
  • Tag / Fusion Protein
    • 6xHis tag + TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCGTCCGGCGTAGAGGATCGAGATCT
  • 3′ sequencing primer CAGCTTCCTTTCGGGCTTTGTTAGCAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-HT_ygiF-TTM was a gift from Michael Hothorn (Addgene plasmid # 127794 ; http://n2t.net/addgene:127794 ; RRID:Addgene_127794)
  • For your References section:

    Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun. 10.26508/lsa.201900385 PubMed 31133615