Addgene - pTGPD-H-Hsp82 Plasmid Data

Plasmid 1278: pTGPD-H-Hsp82

Gene/insert name:
Hsp82
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
HSP82 (YPL240C, HSP90)
Fusion protein or tag:
Histidinex6
Terminal:
N terminal on insert
Vector backbone:
pRS314
(Search Vector Database)
Vector type:
Yeast Expression
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI/NotI
Site destroyed during cloning:
Yes
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Conservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae. Chang et al (J Biol Chem 1994 Oct 7;269(40):24983-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1278" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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