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pTGPD-H-Hsp82
(Plasmid #1278)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Hsp82
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)
  • Promoter GPD
  • Tag / Fusion Protein
    • Histidinex6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI/NotI (destroyed during cloning)
  • 5′ sequencing primer GPDpro-F
  • 3′ sequencing primer M13pUC-Fwd
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTGPD-H-Hsp82 was a gift from Susan Lindquist (Addgene plasmid # 1278)
  • For your References section:

    Conservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae. Chang HC, Lindquist S. J Biol Chem 1994 Oct 7;269(40):24983-8. PubMed 7929182