pMJA056
(Plasmid #128516)

• Purpose: Expresses Xylanase B+GUS
• Depositing Lab: Marcos Alcocer
• Publication: Alcocer et al Appl Microbiol Biotechnol. 2003 Feb;60(6):726-32. doi: 10.1007/s00253-002-1184-4. Epub 2002 Dec 21.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 5458
• Vector type: Bacterial Expression
• Selectable markers: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xylanase B + uidA
• Alt name: AJ489605.1
• Species: Talaromyces funiculosus
• Insert Size (bp): 924
• Promoter: Histone 4B

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: ATTACGCCAAGCTCGAAATTAACCCT
• 3′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pMJA056

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

expression of Xylanase B fused to GUS reporter gene