pIIS18-SapI
(Plasmid
#128599)
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Purpose(Empty Backbone) Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC18
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Backbone manufacturerself-made
- Backbone size (bp) 2556
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Modifications to backboneIt carries a new multiple cloning site (MCS) , out side of the MCS is free from any Type IIS enzyme cleavage site
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Vector typeUnspecified
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Selectable markerslacZ alpha
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIIS18-SapI was a gift from Tohru Suzuki (Addgene plasmid # 128599 ; http://n2t.net/addgene:128599 ; RRID:Addgene_128599) -
For your References section:
A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes. Altaib H, Ozaki Y, Kozakai T, Badr Y, Nomura I, Suzuki T. Microbiol Resour Announc. 2019 Oct 10;8(41). pii: 8/41/e00323-19. doi: 10.1128/MRA.00323-19. 10.1128/MRA.00323-19 PubMed 31601653
Map uploaded by the depositor.
