pETM6_Ptrc_BsMdh_BmHps_BmPhi
(Plasmid
#129104)
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PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepETM6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBsMDH BmHPS BmPHI
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SpeciesBacillus subtilis and Bacillus methanolicus
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GenBank IDBSU_29120, PB1_00480, PB1_00485
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM6_Ptrc_BsMdh_BmHps_BmPhi was a gift from Eleftherios Terry Papoutsakis (Addgene plasmid # 129104 ; http://n2t.net/addgene:129104 ; RRID:Addgene_129104) -
For your References section:
Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph. Bennett RK, Gonzalez JE, Whitaker WB, Antoniewicz MR, Papoutsakis ET. Metab Eng. 2018 Jan;45:75-85. doi: 10.1016/j.ymben.2017.11.016. Epub 2017 Dec 5. 10.1016/j.ymben.2017.11.016 PubMed 29203223
