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pNCST-AausFP1
(Plasmid #129499)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 129499 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNCST
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AausFP1
  • Alt name
    Aequorea cf. australis FP1 - super bright green fluorescent protein
  • Species
    Aequorea cf. australis
  • Promoter synthetic constitutive (stationary phase) promoter + T7 promoter upstream

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Expression from this vector is best in fast-growing E. coli strains.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCST-AausFP1 was a gift from Nathan Shaner (Addgene plasmid # 129499 ; http://n2t.net/addgene:129499 ; RRID:Addgene_129499)
  • For your References section:

    Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing. Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, Nero Moffatt J, Salih A, Royant A, Shaner NC. PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936. eCollection 2020 Nov. 10.1371/journal.pbio.3000936 PubMed 33137097