pRSET Nucleolin
(Plasmid #13037)

• Depositing Lab: Peter Newburger
• Publication: Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Backbone

• Vector backbone: pRSET-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Preferred growth strain is BL21(DE3) for high protein expression.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nucleolin
• Alt name: C23
• Alt name: FLJ 45706
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• GenBank ID: NM_005381
• Entrez Gene: NCL (a.k.a. C23, Nsr1)
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry