Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||13055||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Spectinomycin
Growth instructionsTP997 is a bacterial strain and does not contain a plasmid. It grows at 37 degrees.
Insert Size (bp)600
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Please note that TP997 is NOT a plasmid, but a bacterial strain. It does not contain a plasmid.
TP997 is a strain containing a synthetic operon with the aacC1 gene (gentamicin resistance) fused to an artificial ribosome binding sequence and a variant of the synthetic promoter CP6. Strain genotype: MG1655 lacIPdelta::bla-aadA1148 galK::aacC1067.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TP997 was a gift from Anthony Poteete (Addgene plasmid # 13055)
For your References section:Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. Poteete A, Rosadini C, St. Pierre C. BioTechniques. 2006 Sept. 41(3):261-264