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Browse > Anthony Poteete > Poteete et al > TP997
Bacterial strain 13055: TP997

  • aacC1

  • 600

  • Tn1696

  • na
    (Search Vector Database)

  • Bacterial Expression

  • na List of Sequencing Primers

  • Ampicillin, Spectinomycin

  • Unknown

  • 37

  • TP997 is a bacterial strain and does not contain a plasmid. It grows at 37 degrees.

  • Unknown

  • Gentamicin

  • Anthony Poteete

  • MTA

Comments: 

Please note that TP997 is NOT a plasmid, but a bacterial strain. It does not contain a plasmid.

TP997 is a strain containing a synthetic operon with the aacC1 gene (gentamicin resistance) fused to an artificial ribosome binding sequence and a variant of the synthetic promoter CP6. Strain genotype: MG1655 lacIPdelta::bla-aadA1148 galK::aacC1067.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli Poteete et al (BioTechniques. 2006 Sept. 41(3):261-264)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13055" in your Materials and Methods section.

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