36 p flk1
(Plasmid #13061)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    flk1
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    kdrl (a.k.a. CH211-276G21.4, fk52c05, flk, flk-1, flk1, kdr, kdra, vegfr-2, vegfr2, vegfr4, vegr2, wu:fk52c05)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

For riboprobe, digest with EcoRI and use T7 polymerase.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    36 p flk1 was a gift from Nathan Lawson (Addgene plasmid # 13061)
  • For your References section:

    Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, Weinstein BM. Development. 2001 Oct . 128(19):3675-83. PubMed 11585794