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pLenti-CMV-Blast-DNFGFR1-HA
(Plasmid #130887)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 130887 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti CMV Blast DEST
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 17451)
  • Backbone size w/o insert (bp) 9331
  • Total vector size (bp) 8750
  • Modifications to backbone
    Gateway Cloning
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fgfr1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1002
  • Mutation
    Dominant Negative receptor lacking cytosolic catalytic domain. First 1002 bp of mouse Fgfr1c-isoform (variant 3, lacking immunoglobulin (Ig)-like domain I)
  • GenBank ID
    NM_001079909.2
  • Entrez Gene
    Fgfr1 (a.k.a. AW208770, Ea, Eask, FGFR, FGFR-I, FLG, Fgfr-, Fgfr-1, Flt-, Flt-2, Fr, Fr1, Hs, Hspy, MFR, bFGF-R-1, c-fgr)
  • Tag / Fusion Protein
    • HA-tag (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Amplified from a plasmid received from Sabine Werner
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Werner, S., Weinberg, W., Liao, X., Peters, K., Blessing, M., Yuspa, S., Weiner, R., and Williams, L. (1993). Targeted expression of a dominant‐negative FGF receptor mutant in the epidermis of transgenic mice reveals a role of FGF in keratinocyte organization and differentiation. The EMBO Journal 12, 2635-2643.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-Blast-DNFGFR1-HA was a gift from Gian-Paolo Dotto (Addgene plasmid # 130887 ; http://n2t.net/addgene:130887 ; RRID:Addgene_130887)
  • For your References section:

    Dualism of FGF and TGF-beta Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant. Bordignon P, Bottoni G, Xu X, Popescu AS, Truan Z, Guenova E, Kofler L, Jafari P, Ostano P, Rocken M, Neel V, Dotto GP. Cell Rep. 2019 Aug 27;28(9):2358-2372.e6. doi: 10.1016/j.celrep.2019.07.092. 10.1016/j.celrep.2019.07.092 PubMed 31461652