NCH2exp
(Plasmid
#131019)
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PurposeExpression of plant LINC fluorescent fusion protein for cell biology
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepH7WG2
- Backbone size w/o insert (bp) 11546
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNCH2
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SpeciesZea mays (maize)
- Promoter P35S
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Tag
/ Fusion Protein
- eGFP-FLAG-HA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NCH2exp was a gift from Hank Bass (Addgene plasmid # 131019 ; http://n2t.net/addgene:131019 ; RRID:Addgene_131019) -
For your References section:
Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability. McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann K, Bass HW. Front Plant Sci. 2021 Feb 17;12:645218. doi: 10.3389/fpls.2021.645218. eCollection 2021. 10.3389/fpls.2021.645218 PubMed 33679862
