pMET17p-CCG 316
(Plasmid
#131166)
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Purpose(Empty Backbone) For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS316
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Backbone manufacturerSikorski and Hieter, 1992
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Vector typeYeast Expression
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Tag
/ Fusion Protein
- CCG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- CCG (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMET17p-CCG 316 was a gift from Nils Johnsson (Addgene plasmid # 131166 ; http://n2t.net/addgene:131166 ; RRID:Addgene_131166) -
For your References section:
SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space. Dunkler A, Rosler R, Kestler HA, Moreno-Andres D, Johnsson N. Methods Mol Biol. 2015;1346:151-68. doi: 10.1007/978-1-4939-2987-0_11. 10.1007/978-1-4939-2987-0_11 PubMed 26542721
