pDONR221_SLC7A2
(Plasmid
#131937)
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PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 131937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 * |
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Backbone
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Vector backbonepDONR221
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 2550
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC7A2
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Alt nameCAT-2, HCAT2, ATRC2
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SpeciesH. sapiens (human)
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Insert Size (bp)1974
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Entrez GeneSLC7A2 (a.k.a. ATRC2, CAT2, HCAT2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_SLC7A2 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 131937 ; http://n2t.net/addgene:131937 ; RRID:Addgene_131937)