pDONR221_SLC6A3
(Plasmid #132160)

• Purpose: Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
• Depositing Labs: RESOLUTE Consortium, Giulio Superti-Furga
• Publication: Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10.1038/s41598-020-79218-w.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 2550
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC6A3
• Alt name: DAT, DAT1
• Species: H. sapiens (human)
• Insert Size (bp): 1860
• Entrez Gene: SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-fwd
• 3′ sequencing primer: M13-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information on the full Resolute plasmid collection, please see www.addgene.org/depositor-collections/re-solute/. Please provide your feedback on this plasmid to the RESOLUTE consortium by filling out their RESOLUTE repository feedback form: https://forms.office.com/Pages/ResponsePage.aspx?id=0e05yklzmkS7rjFGQL4N7z4feCLQvEJAmVcOCM_u885UN1JJRko0Ukg4TVQwNTZLOUxPQVJWT1NHUCQlQCN0PWcu