Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

piHGpd/P82
(Plasmid #1325)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1325 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS303 (based on pBluescript
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GPD promoter-HSP82
  • Alt name
    Yeast Heat shock protein 82
  • Alt name
    HSP82
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3800
  • Mutation
    Wild type gene
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid maintained in DH5alpha.
ClaI-NotI fragment from pTGpd/P82 was cloned into pRS303

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piHGpd/P82 was a gift from Susan Lindquist (Addgene plasmid # 1325 ; http://n2t.net/addgene:1325 ; RRID:Addgene_1325)
  • For your References section:

    In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Nathan DF, Vos MH, Lindquist S. Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56. 10.1073/pnas.94.24.12949 PubMed 9371781