PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||132664||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 17500
- Total vector size (bp) 20000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Insert Size (bp)2100
Mutationplease see depositor comments below
- Promoter p1,p3
- Cloning method Gibson Cloning
- 5′ sequencing primer TCCCTACCGATGAATTCGAG
- 3′ sequencing primer TAATTGACAACAGTTAGAAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene found mutations Q291P, P416T, P595S, H623D in mobA during our quality control process. The depositing lab confirmed these mutations do not affect plasmid function, and the plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM5505 was a gift from Susan Golden (Addgene plasmid # 132664 ; http://n2t.net/addgene:132664 ; RRID:Addgene_132664)
For your References section:Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting. Bishe B, Taton A, Golden JW. iScience. 2019 Oct 25;20:216-228. doi: 10.1016/j.isci.2019.09.002. Epub 2019 Sep 10. 10.1016/j.isci.2019.09.002 PubMed 31585408