Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

N4177 strain
(Bacterial strain #13333)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 13333 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    coumermycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Grow at 32oC. Unable to grow at 42oC.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Species
    This is a strain, not a plasmid
  • Mutation
    genotype: strA galK gyrB221 (CouR) gyrB203 (ts). CouR mutation is Arg136Cys. ts mutation is Pro171Ser.

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The strain was derived as follows: a culture of E. coli N99 galK strA was mutagenized with 1-methyl-3'-nitrosoguanidine and Cou-resistant cells were selected on LB agar containing 25ug/mL Cou and 10mM sodium pyrophosphate. These colonies were then screened for failure to grow on LB agar at 42oC. One such strain, N4175, was used as a donor for P1 transduction into N99. The transductant strain, N4177, is Cou-resistant at 32oC and is unable to grow at 42oC.

GyrB protein partly purified from N4177 cells grown at 32oC, when added to wild-type GyrA protein, yields a DNA gyrase complex whose supercoiling activity is Cou-resistant at 25oC and temperature sensitive (less than 1% of the activity of the normal enzyme at 42oC).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N4177 strain was a gift from Martin Gellert (Addgene plasmid # 13333)
  • For your References section:

    Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling. Menzel R, Gellert M. Cell. 1983 Aug . 34(1):105-13. 10.1016/0092-8674(83)90140-X PubMed 6309403