pGL2-basic mt E2F-luciferase
PurposeExpression of E2F1-mt-luc.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133708||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5597
Vector typeMammalian Expression ; trxn factor bind site
Growth in Bacteria
SpeciesH. sapiens (human)
Mutationthe three binding site copies are mutated form
Entrez GeneE2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
HMS clone ID number: HsBS00017288. TATA oligo is GATCTACTTGGGCATAAAGGCAGAGCAGGGCAGCTGCTGCTTACACTTA; 3xE2F-MUTANT oligo is CTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2-basic mt E2F-luciferase was a gift from James A. DeCaprio (Addgene plasmid # 133708 ; http://n2t.net/addgene:133708 ; RRID:Addgene_133708)
For your References section:The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins. Zalvide J, Stubdal H, DeCaprio JA. Mol Cell Biol 1998 Mar;18(3):1408-15. PubMed 9488456