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pGEX-4T-1 EGFP-Sumo2 GG
(Plasmid #13384)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13384 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sumo2 GG
  • Alt name
    SUMO-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    Mature SUMO2GG: diglycine at amino acid positions 92
  • GenBank ID
    NP_008868
  • Entrez Gene
    SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pEGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 EGFP-Sumo2 GG was a gift from Mary Dasso (Addgene plasmid # 13384 ; http://n2t.net/addgene:13384 ; RRID:Addgene_13384)
  • For your References section:

    SUMO-2/3 regulates topoisomerase II in mitosis. Azuma Y, Arnaoutov A, Dasso M. J Cell Biol. 2003 Nov 10. 163(3):477-87. 10.1083/jcb.200304088 PubMed 14597774