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pEGFP(1-233)-mApple(2-236)-HRas(2-189)
(Plasmid #133855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    C3
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5932
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HRas
  • Alt name
    H-Ras
  • Species
    H. sapiens (human)
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • mApple (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Anne Kenworthy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP(1-233)-mApple(2-236)-HRas(2-189) was a gift from Dave Piston (Addgene plasmid # 133855 ; http://n2t.net/addgene:133855 ; RRID:Addgene_133855)

  • For your References section:

    Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins. Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW. Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. 10.1016/j.bpj.2019.09.028 PubMed 31606123
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