Addgene - pFbx15(-332/+114)-Luc Plasmid Data

Plasmid 13407: pFbx15(-332/+114)-Luc

Gene/insert name:
Upstream region of mouse Fbx15 gene
Insert size:
446
Species:
M. musculus (mouse)
Mutation:
None
Vector backbone:
pGV-BM2
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
MunI
Site destroyed during cloning:
Yes
5' sequencing primer:
pGV-2U List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa et al (Mol Cell Biol. 2003 Apr . 23(8):2699-708. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13407" in your Materials and Methods section.