Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSiMPlh_N + pSiMPlh_C
(Plasmid #134316)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 134316 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
  • Alt name
    HPT
  • Species
    Synthetic
  • Mutation
    P254Q in hygR_gp41-1 N-intein

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).

Please see the Sequences link for the individual plasmid sequences.

The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSiMPlh_N + pSiMPlh_C was a gift from Barbara Di Ventura (Addgene plasmid # 134316 ; http://n2t.net/addgene:134316 ; RRID:Addgene_134316)
  • For your References section:

    Split intein-mediated selection of cells containing two plasmids using a single antibiotic. Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B. Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1. 10.1038/s41467-019-12911-1 PubMed 31672972