PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||134405||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2432
- Total vector size (bp) 3445
Vector typeBacterial Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
Growth instructionsWhen transformed with Opto-Cre-Vvd, store in the dark.
Copy numberLow Copy
Insert Size (bp)1013
- Promoter W4 (modified T7A1)
- Cloning method Gibson Cloning
- 5′ sequencing primer ATCTTCCCCATCGGTGATGTCG
- 3′ sequencing primer TTTCGCCACCACTGATTTGAGC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBbAW4k-loxP-TT-loxP-mRFP1 was a gift from Mary Dunlop (Addgene plasmid # 134405 ; http://n2t.net/addgene:134405 ; RRID:Addgene_134405)
For your References section:Light-Inducible Recombinases for Bacterial Optogenetics. Sheets MB, Wong WW, Dunlop MJ. ACS Synth Biol. 2020 Feb 21;9(2):227-235. doi: 10.1021/acssynbio.9b00395. Epub 2020 Jan 21. 10.1021/acssynbio.9b00395 PubMed 31961670