pFA6a-A1:HIS3MX6
(Plasmid
#134427)
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PurposePCR-based C-terminal A1 tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepFA6a-HIS3MX6
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Vector typePCR-based tagging in yeast
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA1
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-A1:HIS3MX6 was a gift from Robert Tomko (Addgene plasmid # 134427 ; http://n2t.net/addgene:134427 ; RRID:Addgene_134427) -
For your References section:
A suite of PCR-based peptide tagging plasmids to permit epitope-targeted enzymatic functionalization of yeast proteins. Nemec AA, Tomko RJ Jr. Yeast. 2020 May 13. doi: 10.1002/yea.3471. 10.1002/yea.3471 PubMed 32401365
