pET15b-tglHI
(Plasmid #134672)

• Purpose: Expresses His6-TglH and TglI in E. Coli
• Depositing Lab: Wilfred van der Donk
• Publication: Ting et al Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232.

Backbone

• Vector backbone: pET15b
• Vector type: Bacterial Expression
• Selectable markers: AmpR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TglH
• Species: Pseudomonas syringae pv. maculicola str. ES4326
• Insert Size (bp): 915
• GenBank ID: EGH61609.1
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminating primer

Gene/Insert 2

• Gene/Insert name: TglI
• Insert Size (bp): 810
• GenBank ID: EGH61610.1
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminating primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry