Addgene - pWay21-MKP-1 FL Plasmid Data

Plasmid 13469: pWay21-MKP-1 FL

Gene/insert name:
MKP-1 FL
Alt name:
MKP-1
Insert size:
1130
Species:
M. musculus (mouse)
GenBank ID:
NM_013642
Entrez Gene:
Dusp1 (3CH134, MKP1, Ptpn16, U19515, erp, mkp-1)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pWay21 EGFP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5800
Cloning site 5':
Sma I
Site destroyed during cloning:
No
Cloning site 3':
Xho I
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Anton Bennett
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation. Wu et al (Mol Cell Biol. 2005 Jun . 25(11):4792-803. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13469" in your Materials and Methods section.