CS2 luc Fstl1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13497||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerSee Rupp et al (1994) Genes Dev 8:1311
- Backbone size w/o insert (bp) 5747
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)147
Mutationcontains 3 tandem copies of the following sequence from the 3'UTR of Fstl1: 5'-acactgagtaccagagggcaagcccacattcctcttctgctac-3'
Entrez GeneFstl1 (a.k.a. AI316791, AW107808, Fstl, TSC-36)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer 5'-cataaaggccaagaagggcg-3' (in luciferase near BglII site) (Common Sequencing Primers)
See author's map for picture of empty pCS2-luc.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CS2 luc Fstl1 was a gift from Stephen Tapscott (Addgene plasmid # 13497 ; http://n2t.net/addgene:13497 ; RRID:Addgene_13497)
For your References section:MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. Rosenberg MI, Georges SA, Asawachaicharn A, Analau E, Tapscott SJ. J Cell Biol. 2006 Oct 9. 175(1):77-85. 10.1083/jcb.200603039 PubMed 17030984
Map uploaded by the depositor.