pJOE5751.1
(Plasmid
#135083)
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PurposepBR322-based L-rhamnose-inducible vector.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameP-rhaP-BAD-His tag-eGFP
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SpeciesEscherichia coli rhamnose promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE5751.1 was a gift from Josef Altenbuchner (Addgene plasmid # 135083 ; http://n2t.net/addgene:135083 ; RRID:Addgene_135083) -
For your References section:
In vivo and in vitro studies on the carotenoid cleavage oxygenases from Sphingopyxis alaskensis RB2256 and Plesiocystis pacifica SIR-1 revealed their substrate specificities and non-retinal-forming cleavage activities. Hoffmann J, Bona-Lovasz J, Beuttler H, Altenbuchner J. FEBS J. 2012 Oct;279(20):3911-24. doi: 10.1111/j.1742-4658.2012.08751.x. Epub 2012 Sep 11. 10.1111/j.1742-4658.2012.08751.x PubMed 22901074
