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pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
(Plasmid #135176)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135176 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
  • Backbone manufacturer
    addgene Plasmid #35509
  • Modifications to backbone
    Ef1a promoter was exchanged to TRE3G promoter obtained from pTRE3G-ZsGreen1 from Clontech.
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hChR2
  • Alt name
    Channelrhodopsin2
  • Species
    Chlamydomonas reinhardtii
  • Mutation
    E123T and T159C
  • Promoter TRE3G
  • Tag / Fusion Protein
    • EYFP (C terminal on insert) (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    TRE3G
  • Species
    Synthetic

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP was a gift from Tetsuo Yamamori (Addgene plasmid # 135176 ; http://n2t.net/addgene:135176 ; RRID:Addgene_135176)
  • For your References section:

    Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset. Ebina T, Obara K, Watakabe A, Masamizu Y, Terada SI, Matoba R, Takaji M, Hatanaka N, Nambu A, Mizukami H, Yamamori T, Matsuzaki M. Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22844-22850. doi: 10.1073/pnas.1903445116. Epub 2019 Oct 21. 10.1073/pnas.1903445116 PubMed 31636197