pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
(Plasmid #135176)

• Purpose: AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
• Depositing Lab: Tetsuo Yamamori
• Publication: Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22844-22850. doi: 10.1073/pnas.1903445116. Epub 2019 Oct 21.

Backbone

• Vector backbone: pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
• Backbone manufacturer: addgene Plasmid #35509
• Modifications to backbone: Ef1a promoter was exchanged to TRE3G promoter obtained from pTRE3G-ZsGreen1 from Clontech.
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hChR2
• Alt name: Channelrhodopsin2
• Species: Chlamydomonas reinhardtii
• Mutation: E123T and T159C
• Promoter: TRE3G
• Tag / Fusion Protein:
  • EYFP (C terminal on insert) (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: nd

Gene/Insert 2

• Gene/Insert name: TRE3G
• Species: Synthetic

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: nd

Resource Information

• Supplemental Documents:
  • AAV2TRE3DIO_ChRET_EYFP.ape
• A portion of this plasmid was derived from a plasmid made by: This plasmid was modified from addgene plasmid #35509. EF1a promoter of #35509 was replaced by TRE3G promoter of pTRE3G-ZsGreen1 from Clontech. The use of TRE3G is licensed from TETsystems Inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry