pJPS2951
(Plasmid
#135585)
-
PurposeSNR6 has UU at position 105–106 deleted
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepSE358
-
Vector typeYeast Expression
-
Selectable markersTRP1
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namesnR6
-
SpeciesS. cerevisiae (budding yeast)
-
MutationU6Δ2 has UU at position 105–106 deleted
-
Entrez GenesnR6 (a.k.a. snR6)
- Promoter Native
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 fwd (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThis plasmid is based on pSX6 that is deposited in Addgene. pSX6 was used as the parental plasmid for site directed mutagenesis to introduce internal deletions with U6
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJPS2951 was a gift from Jonathan Staley (Addgene plasmid # 135585 ; http://n2t.net/addgene:135585 ; RRID:Addgene_135585) -
For your References section:
Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6. Toroney R, Nielsen KH, Staley JP. Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119. 10.1101/gad.328294.119 PubMed 31558568
