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pPS1117
(Plasmid #1356)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1356 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPS934
  • Backbone size w/o insert (bp) 7300
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SXM1
  • Alt name
    pPS934-SXM1-GFP
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    *
  • Entrez Gene
    SXM1 (a.k.a. YDR395W, KAP108)
  • Tag / Fusion Protein
    • sGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

SXM1 replaces NUF2 of pPS934, CEN plasmid. *Note that there is a D->Y mutation in the SXM1. This mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1117 was a gift from Pamela Silver (Addgene plasmid # 1356 ; http://n2t.net/addgene:1356 ; RRID:Addgene_1356)
  • For your References section:

    Importin/karyopherin protein family members required for mRNA export from the nucleus. Seedorf M, Silver PA. Proc Natl Acad Sci U S A 1997 Aug 5;94(16):8590-5. 10.1073/pnas.94.16.8590 PubMed 9238021