Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1356||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7300
Vector typeYeast Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesS. cerevisiae (budding yeast)
Entrez GeneSXM1 (a.k.a. YDR395W, KAP108)
/ Fusion Protein
- sGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
SXM1 replaces NUF2 of pPS934, CEN plasmid. *Note that there is a D->Y mutation in the SXM1. This mutation is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPS1117 was a gift from Pamela Silver (Addgene plasmid # 1356 ; http://n2t.net/addgene:1356 ; RRID:Addgene_1356)
For your References section:Importin/karyopherin protein family members required for mRNA export from the nucleus. Seedorf M, Silver PA. Proc Natl Acad Sci U S A 1997 Aug 5;94(16):8590-5. 10.1073/pnas.94.16.8590 PubMed 9238021