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pAF123
(Plasmid #135921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135921 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAF111
  • Backbone manufacturer
    Eric Kowarz via Addgene
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pSBtet_Hyg_60508; pCMV(CAT)T7-SB100_34879
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

See bioRxiv preprint: https://doi.org/10.1101/831404 Please see the following article for protocols on how to construct or use this vector: Flies, A. S., Darby, J. M., Murphy, P. R., Pinfold, T. L., Patchett, A. L. & Lennard, P. R. Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins. Bio-protocol under review (2020) https://doi.org/10.21769/BioProtoc.3696.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF123 was a gift from Andrew S. Flies (Addgene plasmid # 135921 ; http://n2t.net/addgene:135921 ; RRID:Addgene_135921)
  • For your References section:

    A novel system to map protein interactions reveals evolutionarily conserved immune evasion pathways on transmissible cancers. Flies A, Darby JM, Lennard PR, Murphy PR, Ong CB, Pinfold TL, Lyons AB, Woods GM, Patchett AL. Science Advances 10.1126/sciadv.aba5031