Addgene - integrin alpha9 DM4 pWZL Blast2 Plasmid Data

Gene/insert name:
integrin alpha 9 DM4
Alt name:
ITGA9
Insert size:
3200
Species:
H. sapiens (human)
GenBank ID:
NM_002207
Entrez Gene:
ITGA9 (RLC, ITGA4L, ALPHA-RLC)
Mutation:
C-terminal deletion mutant missing the last 16 amino acids in the cytoplasmic domain.
Vector backbone:
pWZL Blast2
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
6000
Cloning site 5':
HindIII/SnaBI
Site destroyed during cloning:
Yes
Cloning site 3':
XbaI/SnaBI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Dean Sheppard
Terms and Licenses:
MTA

Comments:

See author's map for additional restriction enzyme sites.

Please see Young et al. (2001), MBC 12:3214 for information regarding which amino acids are removed in this deletion construct.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13614" in your Materials and Methods section.