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ABE8.17-m
(Plasmid #136298)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136298 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBR322, beta-lactamase maintenance, CMV promoter
  • Backbone manufacturer
    none
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 8166
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE8.17-m
  • Insert Size (bp)
    4800
  • Mutation
    D10A in S. pyogenes Cas9, TadA mutations described in manuscript
  • Tag / Fusion Protein
    • C-terminal BPNLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABE8.17-m was a gift from Nicole Gaudelli (Addgene plasmid # 136298 ; http://n2t.net/addgene:136298 ; RRID:Addgene_136298)
  • For your References section:

    Directed evolution of adenine base editors with increased activity and therapeutic application. Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. 10.1038/s41587-020-0491-6 PubMed 32284586