pcDNA3.1_mutNSDHL
(Plasmid #136428)

• Purpose: Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.
• Depositing Lab: Antonio Amelio
• Publication: Getz et al Oral Surg Oral Med Oral Pathol Oral Radiol. 2019 Jul;128(1):60-69. doi: 10.1016/j.oooo.2019.02.015. Epub 2019 Feb 23.

Backbone

• Vector backbone: pcDNA3.1_V5-HisA
• Total vector size (bp): 6604
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human NSDHL with mutations in exon 4 and exon 6
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1131
• Mutation: C>T = A105V; G>C = A182P; G>A = R199H; G>A = G205S
• Entrez Gene: NSDHL (a.k.a. H105E3, SDR31E1, XAP104)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5-His tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry