pB-actin 16 Early
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13711||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameHPV 16 early region
Alt nameHPV 16 genome
SpeciesH. sapiens (human)
Insert Size (bp)4400
MutationIncl. small part of L2
- Cloning method Restriction Enzyme
- 5′ cloning site Sal 1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB-actin 16 Early was a gift from Karl Munger (Addgene plasmid # 13711 ; http://n2t.net/addgene:13711 ; RRID:Addgene_13711)
For your References section:The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R.. J Virol. 1989 Oct . 63(10):4417-21.