pGL2084
(Plasmid
#137726)
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Purpose(Empty Backbone) Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRPA-ISL
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Vector typeRNAi ; Trypanosoma brucei tet inducible stem loop
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Selectable markersHygromycin ; Hyg resistance is only generated by correct recombinantion into T.b.brucei 2T1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)ccdB Survival
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Growth instructionsPlasmid has tendency to recombine due to the inverted repeats and the toxic ccdB genes. if grown at temperatures above 25c. MUST be grown slowly at room temperature.
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypGL2084 is a Gateway adapted version of the original pRPA-ISL vector developed by Alsford and Horn, 2008.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL2084 was a gift from Jeremy Mottram (Addgene plasmid # 137726 ; http://n2t.net/addgene:137726 ; RRID:Addgene_137726) -
For your References section:
Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen. Jones NG, Thomas EB, Brown E, Dickens NJ, Hammarton TC, Mottram JC. PLoS Pathog. 2014 Jan;10(1):e1003886. doi: 10.1371/journal.ppat.1003886. Epub 2014 Jan 16. 10.1371/journal.ppat.1003886 PubMed 24453978
